MOLECULAR BIOLOGY
PROTOCOLS
 On this page you will find some of the molecular biology protocols I (used to) use in the lab. All of these protocols, have been tested, and re-tested (or shall I say used, and re-used). Molecular biology can be tricky, and some people clam that there is a dose of wichcraft involved. I really think that if you are concentrated, carefull and follow "good laboratory practices" molecular biology can be very efficient tool, rather than very efficient "drive-you-to-tears" thing. Happy experimenting...

ELISA for FMuLV p30

Total RNA isolation

Lysis by boiling (fast minipreps)

Mini prep alkaline lysis

Plasmid mini prep for sequencing I

Plasmid mini preps for sequencing II

How to blunt DNA ends

Cat assay

Cat assay (liquid phase)

Glass technique

Maxi prep (CsCL)

Phenol extraction

Transformation of ligation




This page is produced and maintained by Peter
Last updated November 13, 2003 @ 21:51
The views and opinions expressed in this page are strictly those of the page author. The contents of this page have not been reviewed or approved by The University of Mississippi Medical Center.